Image Gallery
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A base scaffold is printed with a hydrophilic photopolymer (a) followed by a second printing step using a resin with an on demand protein-binding surface (b) and a final print with Ormocomp providing an inherent protein-adhesive surface (c). PUBLICATION -
3D reconstruction of a laser scanning microscope (LSM) image stack. The basic scaffold (white) is locally functionalized with the proteins vitronectin (magenta) and laminin (red). The cells on the structure are labeled for actin (green, nucleus in white). PUBLICATION -
Prototype structures used to find optimal design parameters for creating photoreceptor cell scaffolds for retinal regeneration. PUBLICATION -
Close-up view of one photoreceptor cell scaffold for retinal regeneration. PUBLICATION -
Retinal progenitor cells (shown in red) attached to and nesting in the vertical pores of a 3D printed photoreceptor cell scaffold (grey). PUBLICATION -
Polymeric buckyballs (C180 design) aimed for cell growth studies with intricate porous structures. -
High-fidelity replication of a human trabecular bone structure from a high resolution 3D μ-CT scan (left). Bone cell culture on the so-called “Osteoprint” (right). APP.NOTE / PUBLICATION -
Cage-like 3D structures for discrimination between tumorigenic and non-tumorigenic human breast cancer cell lines based on different invasiness. APP.NOTE / PUBLICATION /VIDEO
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Muscle fiber actuated microskeleton made of PEGDA-based hydrogel mixed with PETA. APP.NOTE / PUBLICATION -
3D printed tubular structure mimicking a brain capillary of the blood-brain barrier and used as scaffold for endothelial cells. PUBLICATION -
Model mimicking the blood-brain barrier and consisting of the 3D printed tubular structures and the endothelial cells covering them for drug screening. PUBLICATION -
Micro-scaffold/comb for culturing of biological cells. -
3D printed cell scaffold made of photopolymer. PUBLICATION -
3D reconstruction of a laser scanning microscope (LSM) confocal image stack. Single cardiomyocyte (nucleus in white, actin in green) cultured in a fibronectin-functionalized 3D scaffold (white). PUBLICATION -
3D printed cell scaffold made of photopolymer. PUBLICATION -
3D reconstruction of a laser scanning microscope (LSM) confocal image stack. Cardiomyocytes (nucleus in white, actin in green) cultured in a fibronectin-functionalized (green) 3D scaffold (white). PUBLICATION -
Composite scaffold for cells consisting of one cell-repellent (grey, PEG-DA/PETA) and one cell adhesion promoting material (red, Ormocomp). The two-component scaffold was fabricated by two subsequent printing steps. APP.NOTE / PUBLICATION -
Labelled cardiomyocytes (green) deforming a three-dimensional polymeric micro-environment. PUBLICATION / -
3D reconstruction of a laser scanning microscope (LSM) confocal image stack of a 3D printed cell scaffold made of photopolymer. Cell cytoskeleton labelled with actin (green), structure and nucleus of cell (white). -
3D reconstruction of a laser scanning microscope (LSM) confocal image stack of a 3D printed cell scaffold made of photopolymer. Cell cytoskeleton labelled with actin (green), structure and nucleus of cell (white).
Recent Publications
- Near-Infrared Light-Sensitive Polyvinyl Alcohol Hydrogel Photoresist for Spatiotemporal Control of Cell-Instructive 3D Microenvironments
Qin, X.-H., Wang, X., Rottmar, M., Nelson, B. J., Maniura-Weber, K., Adv. Mater. 2018, DOI: 10.1002/adma.201705564 - A 3D Real-Scale, Biomimetic, and Biohybrid Model of the Blood-Brain Barrier Fabricated through Two-Photon Lithography
Marino, A., Tricinci, O., Battaglini, M., Filippeschi, C., Mattoli, V., Sinibaldi, E., Ciofani, G., Small 2017, DOI: 10.1002/smll.201702959 - Two-photon polymerization for production of human iPSC-derived retinal cell grafts
Worthington, K. S., Wiley, L. A., Kaalberg E. E., Collins, M. M., Mullins, R. F., Stone, E. M., Tucker, B. A., Acta Biomaterialia, 2017, DOI: 10.1016/j.actbio.2017.03.039 - Guiding Cell Attachment in 3D Microscaffolds Selectively Functionalized with Two Distinct Adhesion Proteins
Richter, B., Hahn, V., Bertels, S., Claus, T. K., Wegener, M., Delaittre, G., Barner-Kowollik, C., Bastmeyer, M., Adv. Mater. 2017, 29, DOI: 10.1002/adma.201604342 - Three-dimensional cage-like microscaffolds for cell invasion studies
Spagnolo, B., Brunetti, V., Leménager, G., De Luca, E., Sileo, L., Pellegrino, T., Pompa, P.P., De Vittorio, M., Pisanello F., Scientific Reports 5, DOI: 10.1038/srep10531 - The Osteoprint: a Bio-Inspired Two-Photon Polymerized 3D Structure for the Enhancement of Bone-Like Cell Differentiation
A.Marino, C. Filippeschi, G.G. Genchi, V. Mattoli, B. Mazzolai, and G. Ciofani, Acta Biomaterialia 2014, DOI: 10.1016/j.actbio.2014.05.032 - Multifunctional polymer scaffolds with adjustable pore size and chemoattractant gradients for studying cell matrix invasion
A.M. Greiner, M. Jäckel, A.C. Scheiwe, D.R. Stamow, T.J. Autenrieth, J. Lahann, C.M. Franz, and M. Bastmeyer, Biomaterials 2014 Jan; 35(2):611-9.doi: 10.1016/j.biomaterials.2013.09.095 - Fabrication and Characterization of Magnetic Microrobots for Three-Dimensional Cell Culture and Targeted Transportation
S. Kim, F. Qiu, S. Kim, A. Ghanbari, C. Moon, L. Zhang, B.J. Nelson, and H. Choi, Advanced Materials DOI: 10.1002/adma.201301484